Figure 4

Lung inflammation. Bronchoalveolar lavage from RA, CS, HK-NTHi or CS/HK-NTHi mice was centrifuged and the pellet was used to determine total (A) and differential cell (B and C) counts. Supernatant was used to determine levels of IL-1β (D), TNF-α (E), MCP-1 (F) and KC (G) by ELISA. Data in A to C and D to G respectively represent median with range and mean ± SD calculated from 5–6 mice per group (* different from RA-exposed mice, p ≤ 0.05, ANOVA on ranks in A to C and ANOVA in D to G; # different from CS-exposed mice, p ≤ 0.05, ANOVA on ranks in A to C and ANOVA in D to G; † different from HK-NTHi-exposed mice, ANOVA on ranks in A to C and ANOVA in D to G).