| |
Number of cristae per mitochondrion per section
|
FRQ/L**
|
|---|
| |
0
|
< 3
|
3-6
|
>6
|
Mean ± SD
|
Mean ± SD
|
|---|
|
Control
|
0 (0.0%)
|
0 (0.0%)
|
2 (6.7%)
|
28 (93.3%)
|
2.9 ± 0.3
|
2.2 ± 0.8
|
|
1% CSE
|
0 (0.0%)
|
2 (6.5%)
|
9 (29.0%)
|
20 (64.5%)
|
2.6 ± 0.6
|
1.9 ± 0.6
|
|
2.5% CSE
|
4 (12.5%)
|
4 (12.5%)
|
8 (25%)
|
16 (50%)
|
2.1 ± 1.1*
|
1.7 ± 1.0*
|
|
10% CSE
|
4 (11.8%)
|
5 (14.7%)
|
19 (55.9%)
|
6 (17.6%)
|
1.8 ± 0.9*
|
1.3 ± 0.8*
|
|
12.5% CSE
|
0 (0.0%)
|
5 (14.3%)
|
18 (51.4%)
|
12 (34.3%)
|
2.2 ± 0.7*
|
1.6 ± 0.6*
|
|
-10% CSE
|
1 (3.3%)
|
4 (13.3%)
|
12 (40%)
|
13 (43.3%)
|
2.2 ± 0.8*
|
1.6 ± 0.7*
|
| |
***
|
****
|
****
|
- *p < 0.05 pairwise comparison to 0% CSE (Control).
- **Frequency divided by length.
- ***Estimated by chi-square test (p < 0.001).
- ****Estimated by One-Way ANOVA test (p < 0.001).
- BEAS-2B cells were exposed to various concentrations of cigarette smoke extract (CSE) for 6 months and subjected to EM analysis. Representative images of BEAS-2B cells exposed to: 0% CSE (control), 1% CSE, 2.5% CSE, 10% CSE, 12.5% CSE and -10% CSE are shown in Figure 1. In total of 30–35 mitochondria were analyzed per experimental condition. Significance was determined by chi-square test and One-Way ANOVA.
