Prolonged cigarette smoke exposure alters mitochondrial structure and function in airway epithelial cells

Respiratory Research

Table 3 Frequencies and percentages of number of cristae and number of cristae divided by length

	Number of cristae per mitochondrion per section					FRQ/L**
	0	< 3	3-6	>6	Mean ± SD	Mean ± SD
Control	0 (0.0%)	0 (0.0%)	2 (6.7%)	28 (93.3%)	2.9 ± 0.3	2.2 ± 0.8
1% CSE	0 (0.0%)	2 (6.5%)	9 (29.0%)	20 (64.5%)	2.6 ± 0.6	1.9 ± 0.6
2.5% CSE	4 (12.5%)	4 (12.5%)	8 (25%)	16 (50%)	2.1 ± 1.1*	1.7 ± 1.0*
10% CSE	4 (11.8%)	5 (14.7%)	19 (55.9%)	6 (17.6%)	1.8 ± 0.9*	1.3 ± 0.8*
12.5% CSE	0 (0.0%)	5 (14.3%)	18 (51.4%)	12 (34.3%)	2.2 ± 0.7*	1.6 ± 0.6*
-10% CSE	1 (3.3%)	4 (13.3%)	12 (40%)	13 (43.3%)	2.2 ± 0.8*	1.6 ± 0.7*
	***				****	****

*p < 0.05 pairwise comparison to 0% CSE (Control).
**Frequency divided by length.
***Estimated by chi-square test (p < 0.001).
****Estimated by One-Way ANOVA test (p < 0.001).
BEAS-2B cells were exposed to various concentrations of cigarette smoke extract (CSE) for 6 months and subjected to EM analysis. Representative images of BEAS-2B cells exposed to: 0% CSE (control), 1% CSE, 2.5% CSE, 10% CSE, 12.5% CSE and -10% CSE are shown in Figure 1. In total of 30–35 mitochondria were analyzed per experimental condition. Significance was determined by chi-square test and One-Way ANOVA.

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