Figure 3

Mitochondrial capacity is increased in long-term cigarette CSE exposed BEAS-2B cells. Cells were exposed to 0% CSE, 2.5% CSE, 10% CSE 12.5% CSE (n = 4-5) for 6 months. A) Mitochondrial mass was determined by COXIV as a mitochondrial loading control using western blotting. GAPDH was used as loading control. B) ATP levels were measured by bioluminescence. C) Mn-SOD protein levels were detected by western blotting, related to actin and medians are indicated. Western blot analysis of, D) Complex II, E) Complex III and F) Complex V of the oxidative phosphorylation, related to GAPDH (medians are indicated). p = 0.06, p = 0.08, * = p < 0.05, ** = p < 0.01, *** = p < 0.001 and **** = p < 0.0001 between the indicated values.