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Table 2 Median values and interquartile ranges for pulmonary and myocardial mRNA expression in arbitrary units (A.U.)

From: Aggravation of myocardial dysfunction by injurious mechanical ventilation in LPS-induced pneumonia in rats

  LVt, LPS (−) HVt, LPS (−) LVt, LPS (+) HVt, LPS (+)
  (N = 6) (N = 6) (N = 5) (N = 6)
Pulmonary mRNA expression (A.U.)
HSP70 17*10-3 2.5*10-3 3.8*10-3 16*10-3#
(8.7-26*10-3) (0.050-8.2*10-3) (3.4-6.2*10-3) (8.4-27*10-3)
Myocardial mRNA expression (A.U.)
TLR2 0.10*10-3 0.10*10-3 0.10*10-3 0.25*10-3
(0–0.22*10-3) (0–0.25*10-3) (0.10-0.25*10-3) (0.18-0.55*10-3)
TNFα 0.10*10-3 0.15*10-3 0.20*10-3 0.45*10-3
(0–0.38*10-3) (0.075-1.6*10-3) (0.05-0.35*10-3) (0.10-0.63*10-3)
IL6 0 0.05*10-3 0.10*10-3 0.35*10-3
(0–0.35*10-3) (0–3.1*10-3) (0–7.0*10-3) (0–4.7*10-3)
IL1β 0.45*10-3 1.3*10-3 1.6*10-3 1.9*10-3
(0.075-3.9*10-3) (0.38-15*10-3) (0.65-2.6*10-3) (0.83-4.9*10-3)
CXCL1 17*10-3 12*10-3 21*10-3 119*10-3 ##
  (2.73-57.8*10-3) (3.8-67*10-3) (16-37*10-3) (71-225*10-3)
  1. Relative expression levels of target genes were calculated relative to the housekeeping gene β-actin with the formula 2(Ct (β-actin)- Ct (target gene)). LPS lipopolysaccharide, LVt low tidal volume (Vt) ventilation, HVt high Vt ventilation, HSP70 heat shock protein 70, TLR2 toll like receptor 2, TNF α tumor necrosis factor α, IL6 Interleukin 6, IL1β Interleukin 1β, CXCL1 chemokine (C-X-C motif) ligand 1. The interaction between LPS and HVt ventilation increased pulmonary HSP70 (#, P<0.001) and myocardial CXCL1 mRNA expression (##, P=0.013).