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Table 2 Median values and interquartile ranges for pulmonary and myocardial mRNA expression in arbitrary units (A.U.)

From: Aggravation of myocardial dysfunction by injurious mechanical ventilation in LPS-induced pneumonia in rats

 

LVt, LPS (−)

HVt, LPS (−)

LVt, LPS (+)

HVt, LPS (+)

 

(N = 6)

(N = 6)

(N = 5)

(N = 6)

Pulmonary mRNA expression (A.U.)

HSP70

17*10-3

2.5*10-3

3.8*10-3

16*10-3#

(8.7-26*10-3)

(0.050-8.2*10-3)

(3.4-6.2*10-3)

(8.4-27*10-3)

Myocardial mRNA expression (A.U.)

TLR2

0.10*10-3

0.10*10-3

0.10*10-3

0.25*10-3

(0–0.22*10-3)

(0–0.25*10-3)

(0.10-0.25*10-3)

(0.18-0.55*10-3)

TNFα

0.10*10-3

0.15*10-3

0.20*10-3

0.45*10-3

(0–0.38*10-3)

(0.075-1.6*10-3)

(0.05-0.35*10-3)

(0.10-0.63*10-3)

IL6

0

0.05*10-3

0.10*10-3

0.35*10-3

(0–0.35*10-3)

(0–3.1*10-3)

(0–7.0*10-3)

(0–4.7*10-3)

IL1β

0.45*10-3

1.3*10-3

1.6*10-3

1.9*10-3

(0.075-3.9*10-3)

(0.38-15*10-3)

(0.65-2.6*10-3)

(0.83-4.9*10-3)

CXCL1

17*10-3

12*10-3

21*10-3

119*10-3 ##

 

(2.73-57.8*10-3)

(3.8-67*10-3)

(16-37*10-3)

(71-225*10-3)

  1. Relative expression levels of target genes were calculated relative to the housekeeping gene β-actin with the formula 2(Ct (β-actin)- Ct (target gene)). LPS lipopolysaccharide, LVt low tidal volume (Vt) ventilation, HVt high Vt ventilation, HSP70 heat shock protein 70, TLR2 toll like receptor 2, TNF α tumor necrosis factor α, IL6 Interleukin 6, IL1β Interleukin 1β, CXCL1 chemokine (C-X-C motif) ligand 1. The interaction between LPS and HVt ventilation increased pulmonary HSP70 (#, P<0.001) and myocardial CXCL1 mRNA expression (##, P=0.013).