Differential regulation of cell functions by CSD peptide subdomains

Respiratory Research

Table 5 Differential effects of CSD subdomains on monocyte migration, fibrocyte differentiation, and fibroblast collagen and ASMA expression

Peptide	Monoycte migration			Fibrocyte differentiation		Protein expression in fibroblasts and endothelial cells
Peptide	Normal	TGFβ	SSc	Normal	SSc	Collagen NLF	Collagen SLF	ASMA NLF	ASMA SLF	eNOS End
Control	20.1	99.9	303	30	54	100	200	100	260
CSD	↓	↓↓	↓↓	↓	↓	↓↓	↓↓	No Effect	↓↓	↓
Cav-A	↑↑	↓	No Effect	No Effect	No Effect	No Effect	↑	No Effect	No Effect	No Effect
Cav-B	↑	↓	Not Done	Not Done	Not Done	No Effect	No Effect	No Effect	No Effect	↓
Cav-C	↑	↓	Not Done	Not Done	Not Done	↓	↓↓	No Effect	↓	No Effect
Cav-AB	↑	↓	Not Done	Not Done	Not Done	↓↓	↓↓	No Effect	No Effect	↓
Cav-BC	↑	↓↓	↓	↓	↓	↓↓	↓↓	↓↓	↓↓	↓

The Control Peptide row shows the baseline values for each cell type for Monocyte Migration (in Cells/hpf from Figure 2 and Table 3), for Fibrocyte Differentiation (in Elongated cells/field from Figure 7), and for Protein Expression in Fibroblasts (in Arbitrary Units from Figure 10). eNOS expression in Endothelial Cell data are from ref. [11]. The other rows show increases and decreases from these baseline levels caused by CSD and related peptides (↑↑ = > 100% increase, ↑ = 35 to 100% increase, No Effect = 35% decrease to 35% increase, ↓ = 35 to 65% decrease, ↓↓ = 65 to 100% decrease.

ISSN: 1465-993X