Figure 5

Effects of TGFβ, CSD, and its subdomains on F-actin and CXCR4 expression in normal monocytes. As described in the Methods, normal monocytes were isolated, plated on coverslips, treated with the indicated reagents (TGFβ, CSD, or its subdomains), fixed, and stained for CXCR4 and F-actin and with the nuclear stain DAPI. Representative images are shown selected from 20 to 60 cells observed from four donors in each category. (A) Normal monocytes treated with TGFβ and CSD; (B) Normal monocytes treated with TGFβ and CSD subdomains. The Antennapedia Internalization Sequence alone was routinely used as the Control peptide; when tested scrambled CSD attached to the Antennapedia Internalization Sequence gave similar results.