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Figure 13 | Respiratory Research

Figure 13

From: Differential regulation of cell functions by CSD peptide subdomains

Figure 13

Effects of mutated CSD on monocyte and fibroblast functions. The activity of CSD and mutated forms (92A and 90-92A) were compared. (A,B) Monocytes. (C-F) Fibroblasts. (A) Monocyte migration experiments were performed with Normal monocytes and TGFβ-treated Normal monocytes and the indicated peptides (0.1 μM) as described in the Methods. Indication of statistical significance for TGFβ-treated Normal monocytes with Control peptide is versus Normal monocytes with Control peptide. Indications of statistical significance for TGFβ-treated Normal monocytes treated with CSD, 92A, or 90-92A is versus TGFβ-treated Normal monocytes treated with Control peptide. (B) Fibrocyte differentiation experiments were performed with Normal monocytes and the indicated peptides (0.1 μM) as described in the Methods. (C) The levels of collagen I in the culture medium and of pMEK, pERK, ASMA, and actin (loading control) in the cell layer were determined by Western blotting as described in the Methods using NLF and SLF treated with the indicated peptides as described in the legend to Figure 10. (D-G) Densitometric quantification combining data from three experiments similar to (C) performed with three independent pairs of NLF and SLF. (D) Collagen I. (E) pMEK. (F) pERK. (G) ASMA. Indications of statistical significance for SLF plus Control peptide are versus NLF plus Control peptide. Indications of statistical significance for NLF plus 92A, 90-92A, or CSD are versus NLF plus Control peptide. Indications of statistical significance for SLF plus 92A, 90-92A, or CSD are versus SLF plus Control peptide.

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