Figure 3

CCD-8Lu paracrine stimulation of AEC wound repair in vitro. (A) AEC wound repair after 24 hours with SF (black bar) and 0.2% FBS supplemented (grey bar) CCD-8Lu CM. Negative controls represent wound repair with SF-DMEM (black bar) and 0.2% FBS supplemented DMEM (grey bar). Positive control represent wound repair with 10% FBS supplemented DMEM (black bars = positive control for SF-media treated samples; grey bars = positive control for 0.2% FBS supplemented treated samples). (n = 12; ***p < 0.001). (B) AEC proliferation (by MTT assay) after 24 hours of wounding treated with SF (black bars) and 0.2% FBS supplemented CCD-8Lu CM (grey bars). Negative controls represent AEC proliferation after 24 hours with SF or 0.2% FBS supplemented DMEM and positive control represents AEC proliferation with 10% FBS supplemented DMEM (open bar). (n = 4; ***p < 0.001). (C) Representative inverted light microscopic images of AEC wound repair. Negative control = SF-DMEM, Positive control = 10% FBS supplemented DMEM. Data presented as mean ± SD. ns = not significant. Scale bar, 200 μm.