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Figure 2 | Respiratory Research

Figure 2

From: Mesenchymal stem cells promote alveolar epithelial cell wound repair in vitro through distinct migratory and paracrine mechanisms

Figure 2

hMSC paracrine stimulation of AEC and SAEC wound repair in vitro. (A) AEC (grey bars) and SAEC (black bars) wound repair after 24 hours with SF-MSC CMDMEM and SF-MSC CMSABM respectively. Negative controls indicates SF-DMEM for AEC and SF-SABM basal media for SAEC. Positive control represent wound repair with 10% FBS supplemented DMEM for AEC and SAGM for SAEC (AEC, n = 8; SAEC, n = 12; ***p < 0.001). (B) AEC wound repair after 24 hours with hMSC conditioned media and DMEM supplemented with different concentration of FBS (n = 8; ***p < 0.001 vs DMEM). (C) Inverted light microscopic images of SAEC wound repair. Negative and positive controls indicate serum-free SABM and complete SAGM, respectively. (D) Inverted light microscopic images of AEC wound repair. (E) Representative inverted light microscopic images of AEC wound margins after 24 hours in SF-DMEM, SF-MSC CMDMEM and 10% FBS supplemented DMEM. Numbers of migrating AECs were observed at the wound margins of SF-MSC CM (arrows) and DMEM + 10% FBS treated samples. (F) The internuclear distances of AECs at wound margins after 24 hours; SF-MSC CMDMEM (grey bar), DMEM + 10% FBS (open bar), and SF-DMEM (black bar) (n = 6; ***p < 0.001 vs SF-DMEM). (G) AEC proliferation (by MTT assay) after 24 hours of wounding treated with SF (black bars) and 0.2% FBS supplemented (grey bars) DMEM and MSC-CM, and 10% FBS supplemented DMEM (open bar). (n = 4; ***p < 0.001). Data presented as mean ± SD; ns = not significant. Scale bars 200 μm (C and D) and 100 μm (E).

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