Figure 2From: Mesenchymal stem cells promote alveolar epithelial cell wound repair in vitro through distinct migratory and paracrine mechanismshMSC paracrine stimulation of AEC and SAEC wound repair in vitro. (A) AEC (grey bars) and SAEC (black bars) wound repair after 24 hours with SF-MSC CMDMEM and SF-MSC CMSABM respectively. Negative controls indicates SF-DMEM for AEC and SF-SABM basal media for SAEC. Positive control represent wound repair with 10% FBS supplemented DMEM for AEC and SAGM for SAEC (AEC, n = 8; SAEC, n = 12; ***p < 0.001). (B) AEC wound repair after 24 hours with hMSC conditioned media and DMEM supplemented with different concentration of FBS (n = 8; ***p < 0.001 vs DMEM). (C) Inverted light microscopic images of SAEC wound repair. Negative and positive controls indicate serum-free SABM and complete SAGM, respectively. (D) Inverted light microscopic images of AEC wound repair. (E) Representative inverted light microscopic images of AEC wound margins after 24 hours in SF-DMEM, SF-MSC CMDMEM and 10% FBS supplemented DMEM. Numbers of migrating AECs were observed at the wound margins of SF-MSC CM (arrows) and DMEM + 10% FBS treated samples. (F) The internuclear distances of AECs at wound margins after 24 hours; SF-MSC CMDMEM (grey bar), DMEM + 10% FBS (open bar), and SF-DMEM (black bar) (n = 6; ***p < 0.001 vs SF-DMEM). (G) AEC proliferation (by MTT assay) after 24 hours of wounding treated with SF (black bars) and 0.2% FBS supplemented (grey bars) DMEM and MSC-CM, and 10% FBS supplemented DMEM (open bar). (n = 4; ***p < 0.001). Data presented as mean ± SD; ns = not significant. Scale bars 200 μm (C and D) and 100 μm (E).Back to article page