Figure 4

Effects of AUF-1-targeting siRNA on CXCL8 protein expression in HBE cells. HBE cells were treated for 24 h prior to harvesting cell supernatants for analysis with ELISA and whole cell lysates for analysis with immunoblotting. To determine the level of AUF-1 protein knock-down membranes were probed with a specific AUF-1 antibody recognizing 4 isoforms of AUF-1, then were subsequently stripped and re-probed with an antibody to GAPDH to ensure equal protein loading (A; representative of 3 separate experiments). Densitometry analysis with % knock-down relative to HRV+CSE alone is also shown for each isoform of AUF-1 (A; n=3). Supernatants were analyzed for CXCL8 protein (B; n=3). Data are presented as mean ± SEM. Lipid denotes transfection reagent alone. NT denotes non-targeting control siRNA. ns = not significant.