Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons

Table 5 Primers (RT-PCR and qPCR)

Gene/variant Symbol	Forward Primer	Reverse primer	Size (bp)
CEACAM1-4L	AAGACGATCATAGTCACTGAGCT	GGAGACTGAGGGTTTGTGCT	483
CEACAM1-4S	AAGACGATCATAGTCACTGAGCT	ATTGGAGTGGTCCTGAGCTG	454
CEACAM1-3L	TCATAGTCACTGATAATGCTCTACC	GGAGACTGAGGGTTTGTGCT	188
CEACAM1-3S	TCATAGTCACTGATAATGCTCTACC	ATTGGAGTGGTCCTGAGCTG	159
CEACAM1-4C1	AAGACGATCATAGTCACTGAGCT	TTGCACACCATTGACAGAGT	369
CEACAM1-3	CAGTGACCCAGTCACCTTGA	TGGACTTGTTTGTGCCTGTTG	403
CEACAM1-3C2	CAAGACGATCATAGTCACTGAGTC	AGAGGGACATATAGGAAGGGGT	210
CEACAM5	AGGCCAATAACTCAGCCAGT	GGGTTTGGAGTTGTTGCTGG	104
CEACAM6	TCAGCCACTGGCCTCAATAG	TCTGGTCCAATCTGCCAGTC	177
HPRT1	GACCAGTCAACAGGGGACAT	AACACTTCGTGGGGTCCTTTTC	195
PPIB	ATGTAGGCCGGGTGATCTTT	TGAAGTTCTCATCGGGGAAG	219
IFNβ-1a	TGCTCTCCTGTTGTGCTTCT	CCACAGGAGCTTCTGACACT	103

Structure of forward and reverse primers (5′-3′) of all target genes and isoforms and the expected size of their respective amplified PCR-products.

ISSN: 1465-993X