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Figure 5 | Respiratory Research

Figure 5

From: Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons

Figure 5

Regulation of CEACAM expression by M. catarrhalis and non-typable H. influenzae (NTHi). Confluent NHBE cells were incubated with M. catarrhalis strains 25238 (wild type, A), BBH18 (wild type, B), BBH18.1 (UspA1 deletion mutant unable to bind CEACAM1, C), or NTHi strains 2019 (wild type, D), 1128 (wild type, E), 1128f- (P5 deletion mutant unable to bind to CEACAM1, F) for 24 h. Cells were then subjected to qPCR analysis of CEACAM1-4L, CEACAM1-3L, CEACAM1-4S, CEACAM1-3S, CEACAM5, and CEACAM6 mRNA expression. (G) Confluent NHBE cells were incubated with M. catarrhalis strain 25238 or NTHi strain 2019 for 4 h, 8 h or 24 h and subjected to qPCR analysis for IFNβ mRNA expression. (A-G) Distrubution of the normalized expression ratios are represented by box-whiskers plots. Dashed lines represent the median expression; boxes and whiskers represent the interquartile range and the maximum and minimum observation, respectively. *P < 0.05, # P = 0.088, §P = 0.079.

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Respiratory Research

ISSN: 1465-993X

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