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Figure 4 | Respiratory Research

Figure 4

From: Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons

Figure 4

Cell surface expression of CEACAM1, CEACAM5 and CEACAM6 in NHBE cells. (A) Confocal images of indirect immunofluorescences of CEACAM1, CEACAM5 and CEACAM6 in non-permeabilized NHBE cells using mono-specific antibodies. Actin was detected using TRIC-conjugated phalloidin and nuclei were stained with Hoechst33342. Merged images display pseudo-colors; green: CEACAMs, red: actin, blue: chromatin. (B) FACS analysis of CEACAM1 expression on NHBE cell surfaces. Confluent NHBE cells were left untreated or incubated for 24 h with polyI:C, IFNα, IFNβ or IFNγ, respectively, and subjected to FACS analysis. Data are representative of three experiments with similar results. Filled histograms: IgG control, open histograms: CEACAM1. Numbers give mean intensity (arbitrary units). Mean IgG control = 62. (C) FACS analysis of CEACAM5 expression on NHBE cell surfaces. Confluent NHBE cells were left untreated or incubated with IFNγ for 24 h and subjected to FACS analysis. Data are representative of three experiments with similar results. Filled histograms: IgG control, open histograms: CEACAM5. Numbers give mean intensity (arbitrary units). Mean IgG control = 51.

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Respiratory Research

ISSN: 1465-993X

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