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Figure 3 | Respiratory Research

Figure 3

From: Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons

Figure 3

Regulation of CEACAM expression by CSE, cytokines, and TLR agonists. (A) Confluent NHBE cells were tested for the expression of CEACAM1-4L, CEACAM1-3L, CEACAM1-4S, CEACAM1-3S, CEACAM5, CEACAM6 and the house-keeping gene HPRT1 by RT-PCR using the primers listed in Table 5. (B, D, E) Confluent NHBE cells were incubated with IFNα, IFNβ, IFNγ, TNFα, MALP-2, poly I:C, flagellin, or CSE, respectively, for 24 h. Real time qPCR was then used to analyze these cells for CEACAM1-4L, CEACAM1-4S, CEACAM1-3L and CEACAM1-3S mRNA expression (B), CEACAM5 mRNA expression (C) or CEACAM6 mRNA expression (D), respectively. (E) Confluent NHBE cells were incubated with poly I:C for 4, 8 and 24 h and were tested by qPCR for IFNβ mRNA expression. (B-E) The mRNA expression of each transcript was normalized against two housekeeping genes. Expression ratios relative to unstimulated cells were calculated using REST2009 and are represented by box-whisker plots. Dashed lines represent the median expression; boxes and whiskers represent the interquartile range and the maximum and minimum observation, respectively. *P < 0.05, #P = 0.124.

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