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Figure 2 | Respiratory Research

Figure 2

From: Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons

Figure 2

Soluble CEACAMs in bronchoalveolar lavage fluid and NHBE cells. (A) Bronchoalveolar lavage fluid from 181 donors was tested for presence of soluble CEACAM1, CEACAM5 and CEACAM6 by colorimetric sandwich ELISA using specific antibodies. The diagram shows mean CEACAM concentrations (ng/ml) and standard error of positive samples (CEACAM1=4, CEACAM5=142 and CEACAM6=181 positive samples; logarythmic scale; SD in the Results text). Percentages below columns give percent of BALF samples positive for the respective CEACAM. (B) Confluent NHBE cells were tested for the expression of soluble CEACAM1 isoforms CEACAM1-4C1, CEACAM1-3 and CEACAM1-3C2 by RT-PCR. HPRT1 mRNA expression is shown as control housekeeping gene. (C) NHBE cell culture supernatants were tested for the presence of soluble CEACAM1 (approx. 110 kDa), CEACAM6 (approx. 90 kDa) and CEACAM5 (approx. 180 kDa) by immunoprecipitation with specific monoclonal antibodies (B3-17, 2H8-5, and 13H10) or an IgG1 isotype control and subsequent Western blot analysis with polyclonal anti-CEACAM5 antibody that cross-reacts with all three CEACAMs.

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Respiratory Research

ISSN: 1465-993X

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