Figure 8
From: Pyocyanin-induced mucin production is associated with redox modification of FOXA2
Restoration of FOXA2 expression by GSH attenuates PCN-mediated overexpression of MUC5AC and MUC5B mucins. (A-D) GSH treatment restores the expression of FOXA2 and attenuates the expression of MUC5AC and MUC5B mucins. NCI-H292 cells with or without GSH pretreatment were exposed to PCN (12.5 μg/ml) or sterile water (control) for 24 hr. Nuclear proteins were harvested for Western blots using anti-FOXA2 whereas total protein was probed with anti-MUC5AC or MUC5B antibodies (A) and quantified with densitometry (B-D). The same membranes were stripped and probed with antibody nuclear protein Histone H3 for loading control for FOXA2 and β-actin as loading control for MUC5AC and MUC5B. The experiments were independently repeated three times. Densitometry data represent the mean ± SD from three experiments. *p < 0.05 when comparing GSH-pretreated cells against cells exposed only to PCN. (E-F) GSH represses PCN-mediated induction of MUC5AC and MUC5B gene expression. The expression of mucin genes was analyzed by qRT-PCR. The experiments were performed in triplicates in three independent experiments. The data represent the mean ± SD from three experiments.