Figure 5
From: Pyocyanin-induced mucin production is associated with redox modification of FOXA2
PCN induces the expression of mucins in polarized NHBE and 16HBE airways epithelial cells. (A-D) Induction of MUC5AC mucin expression in polarized NHBE (A) and 16HBE (C) cells cultured at the air-liquid interface by PCN (12.5 μg/ml). Epithelial cells exposed to IL-13 (1 μg/ml) or sterile water (12.5 μl/ml) were used as positive or negative control for MUC5AC induction, respectively. MUC5AC induction by PCN (B) and IL-13 (D) was quantified by the ratio of MUC5AC fluorescence signal (green) divided by the total fluorescence signal of DAPI stained nuclei (blue) using the AxioVision Rel. 4.8 software.