Skip to main content

Table 1 qRT-PCR primers

From: Microarray profiling reveals suppressed interferon stimulated gene program in fibroblasts from scleroderma-associated interstitial lung disease

Gene

Forward primer

Reverse primer

Normalisation genes

HPRT1

TGACACTGGCAAAACAATGCA

GGTCCTTTTCACCAGCAAGCT

YWHAZ

ACTTTTGGTACATTGTGGCTTCAA

CCGCCAGGACAAACCAGTAT

Genes of interest

CXCL10

GAAAGCAGTTAGCAAGGAAAG

ATCCTTGGAAGCACTGCATC

ID1

CCAGAACCGCAAGGTGAG

GGTCCCTGATGTAGTCGATGA

IFITM1

TTCTTGAACTGGTGCTGTCT

ATGAGGATGCCCAGAATCAG

IL11

CCTGTGGGGACATGAACTGT

AGGGTCTGGGGAAACTCG

IRF1

CAGCCCAAGAAAGGTCCTC

TTGAACGGTACAGACAGAGCA

NOX4

CTGCTGACGTTGCATGTTTC

CGGGAGGGTGGGTATCTAA

Serpine 1

GGAAAGGCAACATGACCAG

CAGGTTCTCTAGGGGCTTCC

STAT1

GGATCAGCTGCAGAACTGGT

TTTCTGTTCCAATTCCTCCAA

  1. Shown are the primers, written 5’ to 3’, used for measuring expression levels by qRT-PCR for validation of the microarray results. All primer pairs except for YWHAZ, IRF1, and ID1, are intron spanning.
Back to article page

Respiratory Research

ISSN: 1465-993X

Contact us