Figure 6
Quantitative analysis of the number and ingestion effectiveness of conducting airway neutrophils and APCs. The numbers of conducting airway Ly-6G+ neutrophils (A) and MHC II+ APCs per mm2 of epithelium (B) and the percentages of conidia that were internalized by neutrophils (C) and APCs from the total number of conidia in the region under observation (D) were detected. Data were acquired for OVA/OVA (black bars) or OVA/PBS (open bars) animals at different time points following A. fumigatus conidial application. Mean and SEM are presented for two independent experiments with three and four mice per group, respectively. The difference between OVA/OVA and OVA/PBS groups: ** (p< 0.01), *** (p< 0.001), and ns: not significant. Significant difference between neutrophil numbers and conidia percentages at initial and indicated time points: § (p<0.01).