Figure 4From: Elimination of Aspergillus fumigatus conidia from the airways of mice with allergic airway inflammationInternalization of A. fumigatus conidia in the epithelial compartment of conducting airways. (A) Representative confocal image taken from the proximal ventral segment of the whole-mount airway main axial pathway showing the distribution of MHC II+ epithelial DCs (upper left panel: MHC II+ DCs, yellow; epithelium auto-fluorescence, dark yellow), Ly-6G+ neutrophils (upper right panel, green), A. fumigatus conidia (lower left panel, red), and a merged image (lower right panel) at 4 hours post-conidial application. Scale bar = 20 μm. (B, C) The neutrophil locating in close proximity to the luminal side of the epithelium (arrowhead) and the intraepithelial DC (arrow) are indicated on Z-projection (left panels) or on X-projection (right panels) of the area displayed in (A). (D) The same neutrophil and DC are displayed on the three-dimensional larger magnification image of the region boxed in (A). Scale bar = 20 μm (A, B, C); and 10 μm (D).Back to article page