Table 2 Primary antibodies used for immunohistochemistry
Antigen | Clone * | Supplier | Antigen retrieval | Dilution | Against |
|---|---|---|---|---|---|
CD8 | C8/144B | Dako, Glostrup, Denmark | Low pH # | 1:640 | Cytotoxic T-cells |
PC | |||||
CD11c | 5D11 | Novocastra/Leica, Newcastle Upon Tyne, UK | Low pH # | 1:100 | Myeloid dendritic cells and macrophages |
PC | |||||
CD20 | L26 | Dako | Low pH # | 1:1200 | B-cells |
PC | |||||
CD57 | NK1 | Novocastra/Leica | Low pH # | 1:200 | Natural killer cells and T-cell subsets |
PC | |||||
CD68 | PG-M1 | Dako | Low pH # | 1:600 | Monocytes and macrophages |
PC | |||||
Chemokine ligand 21 (CCL21) | Goat polyclonal | R&D Systems, Abingdon, UK | High pH‡ | 1:3000 | Lymphatic endothelium |
PT | |||||
Chemokine scavenger receptor D6 | Made in-house [23] | Provided by Dr. G.J. Graham | High pH‡ | 1:400 | Lymphatic endothelium, leukocytes |
PT | |||||
Ki-67 | Rabbit polyclonal | Biocare Medical, Concord, CA, USA | Low pH # | 1:200 | Cell proliferation |
PC | |||||
Lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) | Rabbit polyclonal | Abcam, Cambridge, UK | Low pH # | 1:400 | Lymphatic endothelium |
PC | |||||
Podoplanin | D2-40 | Biocare Medical | Low pH # | 1:100 | Lymphatic endothelium |
PC | |||||
Prospero-related homeobox transcription factor (Prox1) | Rabbit polyclonal | Abcam | High pH‡ | 1:800 | Lymphatic endothelial cell nuclei |
PT | |||||
α-smooth muscle actin (α-SMA) | 1A4 | Sigma-Aldrich, Saint Louis, MO, USA | Low pH # | 1:1000 | Smooth muscle cells |
PC |