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Figure 1 | Respiratory Research

Figure 1

From: Increased number and altered phenotype of lymphatic vessels in peripheral lung compartments of patients with COPD

Figure 1

Staining characteristics of immunohistochemical markers for detection of lymphatic vessels in the peripheral lung. LYVE-1 immunoreactivity was present on vessels with (A) lymphatic morphology (brown, arrowheads) and (B) blood vessel morphology (brown). (C-E) Double staining immunohistochemistry for podoplanin (brown, arrowheads) and α-smooth muscle actin (α-SMA, red) revealed podoplanin-immunoreactivity on lymphatic vessels and not on α-SMA-positive blood vessels (V). (F) Double staining immunohistochemistry for podoplanin (red) and Prox1 (brown nuclei, exemplified by arrowhead). Ep, bronchiolar epithelium; LA, lymphoid aggregate. All sections were counterstained with Mayer’s haematoxylin (blue stain). Black endogenous pigment deposition is visible in (A-C). Scale bars: (A-C, E) 50 μm; (D) 100 μm; (F) 25 μm.

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Respiratory Research

ISSN: 1465-993X

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