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Figure 3 | Respiratory Research

Figure 3

From: M2 macrophage polarisation is associated with alveolar formation during postnatal lung development

Figure 3

Macrophages are increased during alveolarisation in the developing mouse lung. Flow cytometric analysis of CSF-1R+ leukocyte (Csf1r-EGFP+CD45+) number (B) and proportion (C) within whole lungs (A) across a timecourse of postnatal development. Representative plots displaying gating of EGFP and CD45 double positive cells at each time point (D). n=4-5 lungs/time point. ‘a’ represents significant difference compared to P1, ‘b’ compared to P5 ‘c’ compared to P7, ‘d’ compared to P14, ‘e’ compared to P21. Asterisks following letters represent level of significance, where *=p<0.05, **=p<0.01, ***=p<0.001. Csf1r-EGFP+ cell localisation (green) in the developing lung at P7 which were confirmed as predominantly macrophages by F4/80 immunolabelling (red), and were composed of both alveolar (E) and interstitial (F) macrophage subpopulations (arrows).

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