Figure 1From: M2 macrophage polarisation is associated with alveolar formation during postnatal lung developmentMacrophages are abundant in developing embryonic lungs. Ex vivo culture of E12.5 embryonic lungs, maintained for 48Â hours on floating polycarbonate membranes at the air-liquid interface, supported continuation of branching morphogenesis (A-C). Wholemount immunofluorescence labelling of the lung epithelium (anti-E-cadherin; blue) and macrophages (anti-F4/80; green) revealed extensive macrophage accumulation within developing lungs (D), and in particular within branch points (E).Back to article page