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Figure 6 | Respiratory Research

Figure 6

From: Human leukocyte antigen-G expression in differentiated human airway epithelial cells: lack of modulation by Th2-associated cytokines

Figure 6

A. Protein expression of six separate dAEC cultures resolved for IL-10R1 and IL-10R2. Lane loading controls resolved for β-actin are shown. B. Phosphorylation of STAT3 in dAEC after treatment with 100 ng/ml IL-10 for up to 4 hr. Lane loading controls resolved for GAPDH are shown. Representative of 3 experiments. C. Expression of HLA-G after treatment with IL-10 treatment as demonstrated by qPCR. dAEC were treated with 1–100 ng/ml of IL-10, or 10,000 U/ml of IFN-β as a positive control, for 24 hr, after which RNA expression was examined by qPCR using G5-specific primers. N = 5 experiments. *, P < 0.02 vs no treatment.

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