Figure 4

Cysteamine treatment reduces airway hypersensitivity. (A-C) WT mice were immunized with OVA plus alum and intranasally challenged with OVA. Mice were injected intraperitoneally with cysteamine (40 mg/kg/day) to inhibit TG2 activity. (A) Representative photographs of lungs from PBS- or cysteamine-treated mice. Sections are stained with hematoxylin and eosin (original magnification, ×200). (B, C) BAL fluid was obtained 48 h after the last challenge with OVA. BAL cells were counted and analyzed by Wright–Giemsa staining. Total (B) and differential cell counts (C) were performed. Data are means ± standard deviations of three independent determinations with BAL cells from n = 5 mice/group.