Figure 6

Impact of pharmacologic MIF antagonism in the mouse MIF TG BPD model as reflected by pulmonary phenotype and BAL total cell counts. NB MIF TG or WT mice were exposed to 100% O₂ for PN1-4, with recovery in RA from PN5-14 (BPD model). From PN1-14, some mice were treated with vehicle 1 or the MIF antagonist, MIF098 (0.04 mg/g/d; dissolved in vehicle 1). Representative photomicrographs of lung histology (H&E stain) of NB MIF TG or WT mice exposed to RA or hyperoxia (BPD model), or given treatment as noted above, at PN14 (6A). The figures are illustrative of a minimum of 3 animals in each group. Alveolar size, as measured by chord length, confirmed features noted on lung histology (6B). Each bar represents the mean ± SEM of a minimum of three animals. BAL total cell count of NB MIF TG or WT mice exposed to RA or BPD model, or given treatment as noted above, at PN14 (6C). Each bar represents the mean ± SEM of a minimum of three animals. MIF TG +: macrophage migration inhibitory factor (over-expressing) transgenic; BPD Model: bronchopulmonary dysplasia mouse model; Vehicle 1: 10% DMSO; MIF Antagonist: MIF098; BAL: bronchoalveolar lavage. #P≤0.01, *P<0.05.