Figure 1

Effect of hyperoxia on MIF expression in NB WT mice lungs and the mouse BPD model. (A) Western blot of MIF protein expression in lungs of NB mice exposed to hyperoxia (as described in “Methods” section) from PN1 to PN4. Control lungs were exposed to RA for 4 PN days. The figure is representative of n=3-4 mice per group. (B) Semi-quantitative RT-PCR of MIF mRNA expression in lungs of NB BPD mouse model and control mice exposed to RA, at PN14. (C) Western blot of MIF protein expression in lungs of NB BPD mouse model and control mice exposed to RA, at PN14. The mouse BPD model was generated (described in “Methods” section), as previously reported [15]. Control mice were exposed to RA for 14 days. The figure is representative of n=3-4 mice per group. β: beta; HYP: hyperoxia; MIF: macrophage migration inhibitory factor; PN: post natal; RA: room air; BPD: bronchopulmonary dysplasia mouse model.