Iloprost treatment did not change migration capacity or proliferative rate in control or COPD fibroblasts. Proliferative rate of distal lung fibroblasts from control subjects (n = 7) and patients with COPD (n = 7) (A). All proliferation experiments are the mean of quintuplicate. Cells were stimulated with iloprost (100 nM) in fibroblast medium containing 0.4% serum pretreated with indomethacin (3 μM). Fibroblast medium containing 10% serum was used as internal control. Migration capacity in distal lung fibroblasts from control subjects (n = 4) and patients with COPD (n = 4), *p < 0.05 (B). All migration assays are the mean of triplicates. Data are presented as median with individual values and median values are represented as a line. Statistical analysis is performed with Mann Whitney test to compare differences between control and COPD fibroblasts and Wilcoxon paired rank sum test is used to analyze differences within the control and COPD fibroblasts after iloprost stimulation.