Figure 5From: Defective alterations in the collagen network to prostacyclin in COPD lung fibroblastsBiglycan production is altered by iloprost in fibroblasts from control subjects but not from COPD patients. Biglycan production in distal lung fibroblasts from control subjects (n = 7; A) and patients with COPD (n = 7; B). Cells were stimulated with iloprost (10, 100 or 1000 nM) and in combination with TGF-β1 (10 ng/ml) in 0.4% fibroblast medium pretreated with indomethacin (3 μM). Changes in biglycan production after TGF-β1 stimulation are expressed as fold change compared to non-stimulated fibroblasts in 0.4% medium from control subjects (C) and COPD patients (D). Data are presented as median with individual values and median values are represented as a line. *p < 0.05; **p < 0.01. #p < 0.05 and ##p < 0.01 indicate significant differences in biglycan production after TGF-β1 stimulation compared to basal levels. $p < 0.05 indicate significant difference in biglycan synthesis after TGF-β1 stimulation between control and COPD fibroblasts. Statistical analysis is performed with ANOVA on ranks followed by a post hoc test to compare differences between control and COPD fibroblasts and within the control and COPD fibroblasts after iloprost and TGF-β1 stimulation.Back to article page