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Figure 2 | Respiratory Research

Figure 2

From: Silencing of STIM1 attenuates hypoxia-induced PASMCs proliferation via inhibition of the SOC/Ca2+/NFAT pathway

Figure 2

The effects of chronic hypoxia on STIM1 mRNA and protein expression. Representative Western blot images (A) and summarized data (B) for STIM1 proteins in distal (>4th generation) intrapulmonary arteries from control rats (N) and rats exposed to hypobaric hypoxia for 21 days (CH). β-actin was used as a loading control. Graph shows protein expression of STIM1 normalized to an average level in N. The results are expressed as the mean ± SEM for five experiments *: P < 0.05 compared to the N group. (C) STIM1 (red) and α-smooth muscle actin (ACTA2) (green) immunofluorescence staining in small pulmonary arteries from N and CH. Nuclei are counterstained with DAPI (blue). Scale bar = 10 μm. The results shown are from a single experiment and are representative of three separate experiments. (D) Analysis of STIM1 mRNA expression in distal (>4th generation) intrapulmonary arteries from N and CH. Parallel amplification of the rat housekeeping β-actin gene was used as an internal control. The results are expressed as the mean ± SEM for five experiments. *: P < 0.05 compared to the N group.

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