Figure 6

SiRNA mediated knockdown of ATF6α and ERp57 decreases HDM-induced airway fibrosis. Representative images and scoring for airways stained with Masson’s trichrome (A & B). * indicates p < 0.05 as compared to their respective PBS controls. # indicates p < 0.05 as compared to their siRNA transfected HDM challenged samples. Representative images stained with an antibody for αSMA (C). Western blot analysis of whole lung lysates for αSMA, FSP1 and β-actin (D). Measurement of collagen (E), * indicates p < 0.05 as compared to their respective PBS controls. # indicates p < 0.05 as compared to their siRNA transfected HDM challenged samples, ns = not significant compared to PBS treated samples.