Quantification of IL-13 modulation of the differentiation of BCi-NS1.1 immortalized airway basal cells. BCi-NS1.1 cells were cultured on air-liquid interface (ALI) in the absence and presence of IL-13. A. TaqMan analysis of cell type specific mRNA markers at ALI day 28, including basal cell markers (KRT5, TP63); secretory cell markers (MUC5AC, MUC5B); goblet cell marker (TFF3); Clara cell marker (CC10); and ciliated cell markers (DNAI1, FOXJ1). Data shown is the average ± the standard error of n = 5 independent experiments (BCi-NS1.1, passage 6–15). Statistics were calculated by 2-tailed Student’s t test. U = untreated; IL-13 = treated with IL-13. B. Western analysis of untreated and IL-13 treated cells of cell type specific markers at ALI day 28. Lane 1 – untreated, lane 2 – IL-13 treated. Shown is the expression of a basal cell marker (TP63); Clara cell marker (CC10) and ciliated cell markers (DNAI1, FOXJ1). GAPDH was used as a loading control. C. Immunofluorescent staining of ALI day 28 membranes for untreated and IL-13 treated cells for MUC5AC (secretory cell, green) and nuclei (blue). Bar = 20 μm. For B and C, all data shown is representative of n = 3 independent experiments.