Figure 7
IL-13 modulation of differentiation of BCi-NS1.1 immortalized airway basal cells. BCi-NS1.1 cells were cultured under differentiation inducing conditions on air-liquid interface (ALI) in the absence and presence of IL-13. A. Transepithelial electric resistance (TER) of the airway epithelia in the absence and presence of IL-13. The resistance (Ohms x cm2) was measured at ALI day 28. Data is presented as the percentage resistance relative to untreated cells and plotted as the average ± the standard error of n = 5 independent experiments (BCi-NS1.1, passage 6–15). B. Morphology of untreated and IL-13 treated cells. Alcian blue staining of ALI day 28 sections of BCi-NS1.1 cells untreated or treated with IL-13. Bar = 20 μm. C. Quantification of differentiation. Shown is Alcian blue staining of ALI day 28 sections of BCi-NS1.1 cells untreated or treated with IL-13. The number of positive ciliated and secretory cells scored. Data is presented for each cell type as percentage relative to the total number of cells. Data shown is the average ± the standard error of n = 5 independent experiments (BCi-NS1.1, passage 6–15). All statistics were calculated by a 2-tailed Student’s t test.