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Figure 2 | Respiratory Research

Figure 2

From: Generation of a human airway epithelium derived basal cell line with multipotent differentiation capacity

Figure 2

Isolation of BCi-NS1.1 clone of the BCi-NS1 immortalized human airway basal cell line. A. Morphology of BCi-NS1.1 clone at passage 18 and passage 44. B. Comparison of the parental and clonal immortalized basal cells by Western analysis of cell type specific markers. Lane 1 – Primary basal cells; lane 2 – BCi-NS1 parental immortalized basal cells, passage 24; and lane 3 – BCi-NS1.1 clonal immortalized basal cells, passage 3. For all cells, shown is the expression of the basal cell markers KRT5 and TP63. GAPDH was used as a loading control. C. Immunohistochemical characterization of cytopreps of BCi-NS1.1 cells (passage 22) with cell-type specific markers: KRT5 (basal cell); TP63 (basal cell); CD151 (basal cell); β-tubulin IV (ciliated cell); MUC5AC (secretory cell); TFF3 (goblet cell); CC10 (Clara cell); chromogranin A (neuroendocrine cell); N-cadherin (mesenchymal cell) and isotype control. Bar = 20 μm.

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Respiratory Research

ISSN: 1465-993X

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