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Figure 2 | Respiratory Research

Figure 2

From: Inhibition of phosphodiesterase 4 modulates cytokine induction from toll like receptor activated, but not rhinovirus infected, primary human airway smooth muscle

Figure 2

(A-D): Effect of piclamilast on: formoterol-induced cAMP, accumulated cAMP, IL-6 and PGE 2 release from ASMCs. Concentration of A) formoterol-induced cAMP (n = 6) in cell lysates were measured after 1 hr pre-treatment with piclamilast (10-8-10-5 M) or corresponding vehicle (10-5-10-2% (v/v) DMSO) followed by 5 minutes of stimulation with formoterol at 10-8 M. Concentration of B) accumulated cAMP (n = 5) in cell lysates were measured after 24 hr pre-treatment with piclamilast (10-8-10-6 M) or corresponding vehicle (10-5-10-3% (v/v) DMSO). Concentrations of C) IL-6 (n = 17) and D) PGE2 (n = 4) in cell supernatants were collected at 24 hrs from ASMCs treated with vehicle (10-3% (v/v) DMSO) or piclamilast (10-6 M). *p < 0.05 between groups is compared to the control or the least concentration of piclamilast as indicated by the horizontal lines.

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Respiratory Research

ISSN: 1465-993X

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