Figure 5

Live cell imaging of LysoTracker uptake in differentiated AT2 cells in vitro . A) Graph showing the colony-forming efficiency of epithelial stem and progenitor cells (CD45^neg CD31^neg EpCAM^pos CD104^pos CD24^low) cultured in media supplemented with (plus) or without (minus) 50 nM LysoTracker (mean ± SEM, n = 6). Representative phase contrast and fluorescence images of epithelial stem/progenitor-derived colonies in CFU-Epi cultures grown with LysoTracker-supplemented (50 nM) media after B-C) 9 days D-E) 11 days and F-G) 13 days of culture.