Figure 3

AT2 lineage analysis of primary PI^negCD45^negCD31^negEpCAM^posLysoTracker^poscells. A) Amplification plot from qRT-PCR of Sftpc expression in LysoTracker^pos AT2 cells. B) Percentage of LysoTracker^pos AT2 cells that were immunopositive for proSP-C (mean ± SEM, n = 3 independent sorts). Representative images of LysoTracker^pos AT2 cells stained with C-D) proSP-C (plus DAPI), E-F) Isotype control (plus DAPI) and G-H) hematoxylin and eosin (H&E).