Figure 1

Subsetting of mouse lung cells with LysoTracker Green DND-26 by flow cytometry. FACS plots showing LysoTracker staining of single viable A) CD45^pos CD31^neg EpCAM^neg cells, B) CD45^neg CD31^pos EpCAM^neg cells, C) CD45^neg CD31^neg EpCAM^pos cells and D) CD45^neg CD31^neg EpCAM^neg cells and E) non-viable PI^pos cells. Gates represent LysoTracker^pos staining based on unstained controls (Additional file 1: Figure S1). F) Histogram of proSP-C (green) staining of EpCAM^pos lung epithelial cells, compared to Isotype control (grey).