Figure 2

Differentiated nhpESC expression profile is similar to primary lung fibroblasts. After directed differentiation, gene expression patterns in NHP-ESC-D match the expression pattern of primate lung fibroblasts and are significantly different from pluripotent NHP-ESC, as determined by gene array analysis. We compared undifferentiated nhpESC, differentiated nhpESC, and primary NHP fibroblasts for expression of the pluripotency genes Lin28, Sox2, and OCT4, as well as the fibroblast gene vimentin. In all assays, gene expression of the differentiated nhpESC was similar to the primary NHP fibroblasts and not the pluripotent parental nhpESC line. Statistical tests used were ANOVA followed by Bonferroni’s post-test. Differences between NHP-ESC and NHP-ESC-D or adult NHP are significant (p < 0.01). NHP-ESC-D and adult NHP cells were not significantly different from one another for the expression of any genes.