Table 2 Primers used for qPCR analysis
Gene name | Target gene | Forward primer | Reverse primer | Amplicon size (nt) |
|---|---|---|---|---|
Epithelial genes | ||||
human E-cadherin | hCDH1 | GCCGAGAGCTACACGTTCA | GACCGGTGCAATCTTCAAA | 88 |
human mucin | hMUC5AC | TTCCATGCCCGGGTACCTG | CAGGCTCAGTGTCACGCTCTT | 200 |
Mesenchymal genes | ||||
human N-cadherin | hCDH2 | CTCCATGTGCCGGATAGC | CGATTTCACCAGAAGCCTCTAC | 92 |
human vimentin | hVIM | GTTTCCCCTAAACCGCTAGG | AGCGAGAGTGGCAGAGGA | 68 |
Transcription factor genes | ||||
human Snail1 | hSNAI1 | GCTGCAGGACTCTAATCCAGA | ATCTCCGGAGGTGGGATG | 84 |
human Snail2 | hSNAI2 | TGGTTGCTTCAAGGACACAT | GTTGCAGTGAGGGCAAGAA | 66 |
human Twist1 | hTWIST1 | AAGGCATCACTATGGACTTTCTCT | GCCAGTTTGATCCCAGTATTTT | 96 |
human Twist2 | hTWIST2 | TCTGAAACCTGAACAACCTCAG | CTGCTGTCCCTTCTCTCGAC | 70 |
human Zeb1 | hZEB1 | GCTAAGAACTGCTGGGAGGAT | ATCCTGCTTCATCTGCCTGA | 79-82 |
human Zeb2 | hZEB2 | AAGCCAGGGACAGATCAGC | CCACACTCTGTGCATTTGAACT | 74 |
Housekeeping gene | ||||
human glyceraldehyde 3-phosphate dehydrogenase | hGAPDH | AGCCACATCGCTCAGACAC | GCCCAATACGACGACCAAATCC | 46 |