Figure 5From: Pharmacological inhibition of GSK-3 in a guinea pig model of LPS-induced pulmonary inflammation: II. Effects on skeletal muscle atrophyGSK-3 inhibition using CHIR99021 prevents TNF-α- or glucocorticoid-induced inhibition of myogenesis. Differentiating C2C12 myoblasts were either cultured in the presence or absence of TNF-α (1 ng/ml), dexamethasone (Dex) (10 μM), LiCl (10 mM) or CHIR99021 (2 μM) or vc (DMSO) for 72 h after which the cells were assessed for (A) morphological changes by staining with May-Grünwald Giemsa (40X magnification, scale bar = 500 μm). (B, C) Next, protein levels of MyLC-1 and GAPDH were determined in whole cell lysates by Western blot analysis. All data shown are representative of 3 independent experiments (means ± SEM, n = 3). *p < 0.05 compared with vc control; # p < 0.05 refers to a difference between indicated conditions.Back to article page