Figure 2

Gating strategy and mDC surface molecule analysis. A (gating strategy): Cells were identified in a FSC/SSC-Plot (first column), and Lineage negative/dim (linneg/dim) cells gated (second column). Among linneg/dim cells, plasmacytoid dendritic cells (pDCs) were identified as CD123 and HLA-DR positive cells (third column), and myeloid dendritic cells (mDCs) as CD11c and HLA-DR positive cells (fourth column). B (analysis of surface molecule expression): Surface molecules on mDCs were quantified in histogram-plots using isotype control antibodies to discriminate between specific (red) and non-specific (grey) antibody staining. The expression of surface molecules was either calculated as the percentage of positive mDCs or as the strength of expression (MFI: mean fluorescence index). The results of one representative participant are shown.