Collagen expression in RAGE TG mice compared to controls. Lungs from Control (Co, A and C) and RAGE TG (B and D) mice were stained for Pico-Red (A and B) to visualize total collagen content and type IV collagen (C and D) and only qualitative decreases were observed. Images were at 100X original magnification. Immunoblotting for type IV collagen using equal aliquots of 10 μg total lung protein revealed markedly decreased total type IV collagen expression in RAGE TG mouse lungs compared to controls (E). *Statistical difference (P ≤ 0.05) with at least three replicates per group.