Figure 1

Schematic diagram of experimental design. Mice were sensitized to OVA by intraperitoneal injection on day 0 and 7, with 20 μg OVA and the adjuvent aluminum hydroxide (Alum). Sham-sensitized mice received PBS in Alum. Mice were challenged with an aerosolized 1% OVA solution in sterile PBS for 30 min on days 14–16, immediately followed by exposure to acrolein (6 hrs; 5 ppm) on days 14–17. Mice were sacrificed on either day 14 (immediately after acrolein exposure; 6 hrs) or on day 18 (48 hrs after the final OVA challenge).