Figure 8

The effect of GW3965 on anti- CD2/3/28 stimulated inflammatory mediator production from PBMCs. PBMCs isolated from the peripheral blood of non-smoking controls (A, C, E, and G) and COPD patients (B, D, F, and H) (n=10 for both groups) were treated with GW3965 (1 μM or 10 μM) (white bars), or dexamethasone (1 μM) (dark grey bars) for 1 h prior to anti-CD2/3/28 stimulation for 24 h. Culture supernatants were analysed for IL-2 (A-B), IL-10 (C-D), IL-13 (E-F), and IL-17 (G- H). Data shown are mean ± SEM. *, **, and *** = significant mediator reduction below vehicle control calculated using absolute values (p<0.05, p<0.01, and p<0.001 respectively).