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Figure 1 | Respiratory Research

Figure 1

From: Role of Abl in airway hyperresponsiveness and airway remodeling

Figure 1

Targeting of the abl gene in smooth muscle cells. (A) SM22-Cre construct possesses a SM22 promoter (PSM22) and a Cre coding region. The abl locus containing a floxed version of the Exon5 of abl and the predicted product of Cre-mediated recombination are also shown. Numbered arrows represent PCR primers. (B) Ethidium bromide-stained agarose gel of PCR products amplified from mouse tails of indicated mouse strains. PCR with primers 3 and 5 demonstrate selective deletion of DNA between loxP sites in the presence of Cre recombinase in vivo. Each lane represents tail DNA samples isolated from an individual mouse of the indicated genotype. (C) Extracts of airway smooth muscle cells from Abl-lox and Ablsm−/− mice were analyzed by immunoblotting. Knockout of the Abl protein is verified in airway smooth muscle. Representative blots from three separate experiments are shown.

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