Effect of IGF-1 on αSMA promoter activity in αSMA-GFP transgenic mice. (A) MLF from αSMA-GFP mice were treated with IGF-1 (100 ng/ml), TGFβ (10 ng/ml), or both on tissue culture plate for 24 hr. Control group was maintained in serum-free media. Representative histogram of each treatment condition overlaying control group histogram is shown. (B) Bar graph summarizing the mean green fluorescence of each treatment condition. 3000 cells/condition analyzed. *p < 0.05 compared to serum-free control.