AF-1 regulated the expression of cyclin and inhibited the growth of NIH3T3. (A). The cells were cultured in medium containing TGF-β1 (10 ng · mL-1) with various concentrations of AF-1 (0–10 μM). The OD values of BrdU incorporation were measured by microplate reader. (B). The cells were cultured in medium containing TGF-β1 (10 ng · mL-1) in the absence or presence of AF-1 (10 μM), the mean fluorescence intensity (MFI) of Cyclin D1 and p27 were determined by flow cytometry. Before statistics and analysis, the data divided by the mean of control group. NC: negative control. n = 3–4. Bars: mean ± SD . *P ≤ 0.05 vs TGF group.