The antifibrotic effects of therapeutic treatment with AF-1 on bleomycin-induced lung fibrosis. (A) The histopathologic examination was determined by H&E staining (upper panel ) and Masson staining (lower panel) (×200, Scale bar = 100 μm). (B). Ashcroft score was used to evaluate the degree of fibrosis. (C). Collagen content was estimated by hydroxyproline assay. n = 5–7. Bars: mean ± SD . *P ≤ 0.05 vs BLM group.